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Central Instrumentation Facility

Welcome to BITS Pilani

Central Instrumentation Facility

 

  • XRD
  • FESEM
  • sif-2
  • CAL Lab
  • SIF Lab

The Central Instrumentation Facility houses multiple high-end and analytical instruments to facilitate research and teaching. The facility is mainly used for research and training purposes. The facility has two components: Sophisticated Instrument Facility (SIF) and Central Analytical Laboratory (CAL), which houses high-end instruments used for research purposes in the field of Science and Engineering. It also includes a high-performance computing (HPC) facility. A team of faculty members and dedicated staff takes care of the facility and its operation. The SIF provides services to both internal and external users on a payment basis. The details of instruments housed under SIF can be accessed at https://discovery.bits-pilani.ac.in/SIF/. Services are booked online by both internal and external users. CAL houses several scientific and analytical instruments that are majorly used for different teaching courses in physics, chemistry, biological sciences, pharmacy, and engineering offered by the institute. 

SIF Facilities

Field Emission Scanning Electron Microscope (FESEM)

Make: FEI

Model: Apreo LoVac

Applications:

  • Topographical morphology & Microstructural analysis of materials including biological cell.
  • Particle size measurement of nanomaterials.
  • Chemical composition analysis of materials with EDS detector.
Fully Spectral Confocal Laser Scanning Microscope (FSCLSM)

Make: Carl Zeiss

Model: LSM 880

Applications:

  • Multi-colour, live/fixed cell and DIC imaging.
  • Z- stacks, 3D image reconstruction, Tile scan.
  • Time series (with or without Z-stack), Co-localization analysis, bleaching, Airyscan, FCS.
  • Topography analysis, surface roughness and Zheight profiling.
Nuclear Magnetic Resonance Spectrometer (NMR)

Make: Bruker

Model: AV NEO (400MHz)

Applications:

  • Analysis of 1D NMR: Proton (1H), Carbon (13C), Fluorine (19F), Phosphorus (31P).
  • Analysis of 2D NMR: COSY, NOESY, HMBC, HSQC.
Gas Chromatography–Mass Spectroscopy (GC-MS/MS )

Make: Shimadzu

Model: TQ8040 CI/NCI

Applications:

  • Qualitative and quantitative analysis of organic compounds.
  • Mechanistic study of fragmentation process under mass spectrometric condition.
  • Molar mass and structural analysis of small biomolecules.
X-Ray Photoelectron Spectrometer (XPS)

Make: Thermo Scientific

Model: K Alpha

Applications:

  • Surface chemistry analysis of the materials
  • Measurement of depth profile.
  • Measuring the energies of the valence states of metallic, semiconducting and adsorbate-covered metal and semiconducting surfaces.
BET-Chemisorption Analyzer

Make: Anton Paar

Model: Autosorb iQ-C-XR-XR-XR

Applications:

  • Measurement of specificsurface area of micro/nano particles.
  • Measurement of pore size, pore volume and pore size distribution of micro/nano particle.
Raman Spectrometer- Atomic Force Microscope

Make: Horiba/AIST-NT

Model: LabRam HR Evolution, Omega Scope

Applications:

Raman:

  • Phase identification of polymorphic solids.
  • Polymer identification, Composition determination.
  • Determination of residual strain and crystallographic orientation.

AFM:

  • Surface morphology at atomic scale.
  • Roughness and grain size measurement of thin films
Single Crystal X-ray Diffractometer (SC-XRD) 

Make: Rigaku

Model: XtaLAB Pro2 Mo

Applications:

  • Measurement of particle size distribution.
  • Measurement of Zeta potential.
Nano-particle Analyzer with Rheometer

Make: Anton Paar

Model: Litesizer 500

Applications:

  • Measurement of particle size distribution.
  • Measurement of Zeta potential.
Impedance Analyzer

Make: Keysight Technologies

Model: E4990A

Applications:

  • Measures impedance against frequency and temperature of materials & nanocomposites.
  • Measurement of dielectric primitivity and conductivity over a large frequency range.
Semiconductor Parameter Analyzer

Make: Convergent Technology

Model: Keithley 4200ASCS

Applications:

  • Advanced measurement hardware for DC I-V, C-V, and pulsed I-V measurement types.

CAL Facilities

3D Bioprinter

Make: ATGC India

Model: INCREDIBLE+

Applications:

  • Tissue engineering: fabrication of complex tissues and organs.
  • Drug development/screening.
  • Toxicology Screening
  • Toxicology Screening
Atomic Absorption Spectrophotometer (AAS)

Make: Shimadzu

Model: AA-6880F

Applications:

  • Analysis of magnesium and calcium in tap water (water analysis).
  • Determination of amount of trace elements in contaminated soil (soil analysis).
  • Clinical analysis: Estimation of metals in biological fluids such as blood, urine, serum, etc.
  • Trace elements analysis in foods, cosmetics, hair, etc.
  • Mining: The amount of metal such as gold can be determined in rocks.
  • Pharmaceuticals: The amount of catalyst used may be present in trace amounts in final pharmaceuticals. Thus, AAS is used to determine the amount of catalyst present.

Atomic Absorption Spectrophotometer (AAS)

Make: Shimadzu

Model: AA-6880F

Applications:

  • Analysis of magnesium and calcium in tap water (water analysis).
  • Determination of amount of trace elements in contaminated soil (soil analysis).
  • Clinical analysis: Estimation of metals in biological fluids such as blood, urine, serum, etc.
  • Trace elements analysis in foods, cosmetics, hair, etc.
  • Mining: The amount of metal such as gold can be determined in rocks.
  • Pharmaceuticals: The amount of catalyst used may be present in trace amounts in final pharmaceuticals. Thus, AAS is used to determine the amount of catalyst present.
Benchtop Nuclear Magnetic Resonance Spectroscopy (NMR)

Make: Inkarp

Model: 60 Prop

Applications:

  • It is used by chemists and biochemists to identify the properties of organic molecules.
  • It is used to analyse mixtures containing known compounds.
  • It is used to determine the molecular structure of different compounds
  • It is used to check the clarity and purity of given samples.
  • It is used in the study of various drugs.
  • It is used in food science.
Benchtop Powder XRD (X-Ray Diffraction)

Make: Bruker

Model: D2 Phaser

Applications:

  • Characterization of crystalline materials.
  • Identification of fine-grained minerals such as clays and mixed layer clays that are difficult to determine optically.
  • Determination of unit cell dimensions.
  • Measurement of sample purity
Bio Fermenter

Make: Genetix

Model: FMT-ST-505 Fermenter

Applications:

  • Production of antibiotics, hormones and enzymes
  • Provide a controlled environment for the growth of microorganism.
Centrivap Concentrator Freeze Dryer

Make: Labquip India

Model: Centrivap

Applications:

  • Centrifugal vacuum concentrators can be applied in residue analysis successfully.
  • Even methanol/water-or acetonitrile/water-mixtures as extracts from food or other samples can be concentrated without separation of water prior to the evaporation process.
  • Sulphonamides and other drugs can be concentrated with 100% recovery.
  • For more volatile substances (such as organochlorine pesticides or polychlorinated biphenyls) centrifugal vacuum concentrators can be used for preconcentration.
  • Evaporate liquid and concentrate DNA, RNA, nucleotides and other proteins for further use or study.
Chemidoc

Make: Bio Rad

Model: Universal-H

Applications:

  • Quantitative analysis of protein and DNA samples.
  • Suitable for laboratories engaged in RNAi analysis,molecular diagnostics, epigenetics, pharmacogenomics and forensic testing of genomic DNA.
Cytoflex

Make: Beckman Coulter

Model: Laser-2

Applications:

  • Determination cell structure and cell function
  • Cytokine expression, DNA content analysis, Apoptosis, ROS generation
  • Stem Cell Identification
Differential Scanning Calorimeter (DSC)

Make: Shimadzu

Model: DSC-60

Applications:

  • Structural phase- transition of a compound can be analysed
  • Melting compound of any compound can be determined
  • Heat of fusion can be analysed
  • Percent of crystallinity and crystallization kinetics can be studied.
  • Thermodynamical analysis of biomolecules can be performed
  • Oxidative stability of particular compound can be checked
Elemental Analyzer

Make: Elementer

Model: Vario Micro

Applications:

  • Detection of carbon, hydrogen, nitrogen, and sulphur analysis, offering additional options for the determination of oxygen, chlorine, and total inorganic carbon
  • Analyzing organic element
  • Analyzing soil
Ellipsometer

Make:

Model:

Applications:

  • Eillipsometer is widely used for thin film analysis and measurements.
Flash Chromatography

Make: Cheetah

Model: Cheetah MP 200

Applications:

  • Pharmaceutical
  • Purification of synthetic compounds
  • Purification of complex mixture of synthetic compounds
  • Isolation of biopolymers such as peptides and nucleotides
  • Separation of combichem arrays
  • Purification of natural products
Flame photometer

Make: Systronics

Model:

Applications:

  • Widely used in chemistry, material science.
  • Used to determine the concentration of certain metal ions like sodium, potassium, lithium, calcium and cesium etc.
Fluorimeter

Make: Shimadzu

Model: RF5301PC

Applications:

  • Direct and indirect analysis of vitamins, proteins, peptides and many drug molecules
  • Unknown location of specific sample can be analyzed in any of the organ if the particular compound shows fluorescence
  • Detection of environmental pollutants
  • In geology, many different types of calcites will show fluorescence
  • Detects compounds that show fluorescence
  • Analysis of proteins and amino acids
FTIR (Fourier Transform Infrared Spectroscopy)-Bruker

Make: Bruker

Model: Alpha

Applications:

  • It is applied for detection and identification of different elements/compounds in solving problems in the fields of pharmaceutical, bio-chemistry, material science etc.
  • Chemical bonds can be detected through their characteristic infra-red absorption frequencies or wavelengths.
  • Structural elucidation by determining the functional group.
  • Identification of geomaterial isomers for both organic and inorganic samples
FTIR (Fourier Transform Infrared Spectroscopy) - Shimadzu

Make: Shimadzu

Model: IR Spirit-T

Applications:

  • It is applied for detection and identification of different elements/compounds in solving problems in the fields of pharmaceutical, bio-chemistry, material science etc.
  • Chemical bonds can be detected through their characteristic infra-red absorption frequencies or wavelengths.
  • Structural elucidation by determining the functional group.
  • Identification of geomaterial isomers for both organic and inorganic samples
Freeze Dryer Centrifuge

Make: labquip India

Model: Triod

Applications:

  • It is widely used in the fields of pharmaceutical, biological-science (e.g., bacteria, yeasts), bio-medical, food-processing etc.
  • Samples for both types of freezes and drying can be run at once.
Gas Chromatography

Make: Shimadzu

Model: GC-2014

Applications:

  • Gas chromatography is vital to the food industry for ensuring the safety of food products.
  • Manufacturing relies on gas chromatography for quality control.
  • Gas chromatography is fundamental to many research areas.
  • For many years gas chromatography has been used in forensic science.
  • Measuring air pollution.
High Performance Liquid Chromatography (HPLC)

Make: Thermofisher

Model: Ultimate-3000

Applications:

  • One of the most common uses of HPLC is in the manufacturing process of pharmaceutical products. HPLC is a reliable and precise way to check product purity.
  • HPLC is also used for the detection of drug traces in urine.
  • HPLC also lends itself to the analysis of nutrients in blood and other medical samples.
  • HPLC is often used to analyse biological samples from people with existing diagnoses.
  • HPLC can also be used to identify and quantify pesticides along with preservatives and artificial flavourings and colorants.
High Performance Thin Layer Chromatography (HPTLC/MS)-Camag

Make: Camag

Model:

Applications:

  • Used in quality control and identifying content uniformity in pharmaceutical industry
  • Natural product identification
  • Drug screening and metabolic studies
  • Stability testing can be performed
  • Identification of additives
  • Environmental impurities can be studied
  • Fingerprint analysis can be carried out.
High Performance Liquid Chromatography with PDA detector

Make: Shimadzu

Model: Prominence LPGE PDA

Applications:

  • One of the most common uses of HPLC is in the manufacturing process of pharmaceutical products. HPLC is a reliable and precise way to check product purity.
  • Used for the detection of drug traces in urine.
  • It also lends itself to the analysis of nutrients in blood and other medical samples.
  • Often used to analyse biological samples from people with existing diagnoses.
  • Used to identify and quantify pesticides along with preservatives and artificial flavourings and colorants.
Inverted Fluorescence Microscope

Make: Carl Zeiss

Model: Axio Vert.A1

Applications:

  • Widely used in pharmacy, biological science
  • Analyses all information frtom gel
  • Reveals even Finest Structures in the Cell Nucleus
Milli Q

Make: Merck

Model: Elix Essential 10

Applications:

  • Production of Millipore water
  • Water purification.
Paper Electrophoresis 1

Make: MAC

Model: MSW-492

Applications:

  • Give important diagnostic information concerning serum proteins.
Polarimeter

Make: Rudolph Research Analytical

Model: AP111-6W-1

Applications:

  • Analyzing chiral substances and determining their concentration in solutions.
  • Quality control, laboratory analytics, and R&D in the pharmaceutical, cosmetics, chemical, food, and medical industries.
  • Remote sensing applications, such as planetary science, astronomy, and weather radar.
  • Determining the purity and concentration of ingredients in pharmaceuticals.
Potentiostat

Make: CH Instrument

Model: CHI 1200C

Applications:

  • Energy conversion applications. Batteries, supercapacitors, fuel cells, solar cells, hydrogen evolution, and other energy conversion applications are developed to store more charge, have higher power, etc.
  • Corrosion research and Electroanalysis.
RF Spectrofluorometer

Make: Shimadzu

Model: RF5301PC

Applications:

  • Measuring Fluorescence Quantum Efficiencies of Quantum Dots
  • Biopharmaceutical Development
  • Measurement of Photon Upconversion Luminescence
  • Measurement of Mineral Fluorescence
  • UPF Evaluation of Fabric Products such as Functional Masks
Spectroflurometer T Anisotropy Fluorolog -3

Make: Horiba

Model: Fluorolog-3

Applications:

  • Automotive & Aeronautics.
  • Semiconductors.
  • Material Sciences.
  • Characterization of cosmetic products.
  • Food & Beverage
Thermal Evaporator

Make: VecSolutions

Model: VEC

Applications:

  • Thin film deposition of metal target including Gold, Silver, Platinum, Copper, Iron to any Substrate.
  • Deposition of conducting layer to any semiconductor, non-conducting substrate
Thermo Gravimetric Analyzer (TGA)

Make: Shimadzu

Model: TGA 50

Applications:

  • Thermal stability of materials.
  • Oxidative stability of the given sample can be studied by oxidation of the sample in air instead of an inert atmosphere.
  • Composition of multi-component systems can be evaluated by studying the nature of thermogram of the sample.
  • Sufficiently different behavior on the temperature scale can be identified and reaction mechanism can be formulated.
  • Lifetime of a product can also be estimated using TGA
Ultra-Fast Liquid Chromatography (UFLC)

Make: Shimadzu

Model: 2010 HT

Applications:

  • Separation and analysis of non-volatile compounds can be done easily even in lower concentration when compared to UV
  • Qualitative and quantitative analysis of pure and mixture of compounds can be done.
Ultraviolet Visible Spectrophotometer (UV-VIS-2600)

Make: Shimadzu

Model: UV-2600

Applications:

  • UV-VIS deals with the purity of nucleic acids.
  • UV-VIS is an indispensable equipment in production of pharmaceutics.
  • UV-VIS is essential in the biomass growth curve studies.
  • UV-VIS is also used in FMCG industries.
UV-VIS (Jasco V750)

Make: Jasco

Model: V-750

Applications:

  • A UV-Visible Spectrophotometer for Quantitative Chromaticity and Turbidity Measurements.
  • Absolute Reflectance Measurement of a Dichroic Mirror using UV-Visible/NIR Spectroscopy.
  • Absolute Reflectance Measurement of a Highly Reflective Material by UV-Visible Spectroscopy.
UV-VIS-NIR

Make: Shimadzu

Model: UV- 3600

Applications:

  • Measurement of band gap in Compound semiconductor
  • Detection of impurities in the given organic sample
  • Structural elucidation of the compound can be done like un-saturation/ presence of hetero atom.
  • Quantitative analysis of any compound that can absorb UV radiation.

Training & Workshops

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CIF Policies

CIF Operational Guidelines

CIF Operational

The Central Instrumentation Facility (CIF) is overseen by a Faculty In-charge and two Co-Coordinators who are appointed by the Director of the institution. Together, they manage the operations of the facility with the support of a team consisting of faculty members and dedicated technical staff. This team works collaboratively to ensure the smooth functioning and efficient operation of the CIF, providing necessary assistance and expertise to users of the facility. The facility is comprised of two distinct wings: Sophisticated Instrumentation Facility (SIF) and Central Analytical Laboratory (CAL). These wings are equipped with specialized instrumentation and equipment to support various research activities and analytical needs.

Centre hosts following two types of facility:

SIF Facility:

The Sophisticated Instrumentation Facility (SIF) primarily serves as a research resource for use by research scholars, faculty members, and external users. Access to the facility is typically on a payment basis, allowing individuals to utilize advanced instrumentation and equipment for their research projects. This arrangement enables researchers to leverage sophisticated tools and technologies to conduct experiments and analyze data, contributing to scientific advancement and innovation.

Equipment operations:

  1. Each equipment will have a designated equipment faculty in-charge (Convener) and a Staff in-charge (Technical Assistant), who serves as the operational owner of the equipment. Operational decisions will primarily be made by the Staff in-charge (SIC). Users must adhere to the instructions provided by the SIC, whose decisions within the lab are final and binding.
  2. Equipment operation will be restricted to designated staff members only.
  3. The SIF staff will enforce a strict "No Exception" policy, ensuring that rules and regulations are uniformly applied to all users and equipment within the facility.

Equipment slot booking:

Slot may be booked by the users through online portal of the facility: https://web.bits-pilani.ac.in/pilani/sif/home.

Usage charge rate: Link with attachment of rate chart

CAL Facility:

The Central Analytical Laboratory (CAL) is primarily utilized by undergraduate and higher degree students in their laboratory courses. This facility serves as a practical learning environment where students gain hands-on experience with analytical techniques and instruments relevant to their field of study. The CAL is instrumental in supporting the academic curriculum of undergraduate programs, providing students with the opportunity to apply theoretical knowledge in a practical setting.

CAL equipment operations:

Few equipment are available for research purpose. Contact details for accessing the equipment at CAL facility is: ic.cal@pilani.bits-pilani.ac.in

Training and outreach of the Facility:

The Centre organizes hands-on training programs and workshops aimed at researchers, higher degree students, and faculty members interested in diverse scientific aspects related to the equipment available. These training sessions and workshops provide participants with practical experience and in-depth knowledge about utilizing the sophisticated instrumentation and tools offered by the Centre. The details of the same can be available at: Training program link

Grievances redressal mechanism:

Grievances, suggestions, and feedback from users will be collected through an online platform. The team will gather and review these submissions, engaging in collective discussions to address concerns and make informed decisions. It's important to note that the CIF will not process verbal communications regarding grievances, suggestions, or feedback. Instead, users are encouraged to utilize the designated online platform or communication channels established by the facility for submitting their inputs. By centralizing feedback through an online system, the CIF aims to streamline communication, ensure transparency, and facilitate efficient resolution of issues raised by users. This approach allows for structured feedback collection and enables the CIF team to prioritize and address matters effectively.

Acknowledgement to CIF

Acknowledgment of the BITS Pilani, Pilani campus, CIF facility is encouraged by the CIF team with following lines:

____ acknowledges the Central Instrumentation Facility (CIF), BITS Pilani, Pilani Campus.We request all users to submit the publication details to the CIF data base

Important Instruction

Please find below the important instructions for utilizing the CIF facility:

  1. The analytical data provided is intended solely for research and development (R&D) purposes and must not be used for any legal disputes under any circumstances.
  2. Users are required to book their slots online through the Central Instrumentation Facility (CIF) portal at https://www.bits-pilani.ac.in/pilani/central-sophisticated-instrumentation-facility/. The detailed instructions for the slot booking process are provided in the attached document. Internal Users External Users
  3. Users are responsible for coordinating with the Staff-in-charge(SIC) before analysis and providing all relevant information related to the sample. Users should arrange to meet and discuss at a time convenient for the technical staff to ensure that ongoing sample analyses are not disrupted.
  4. Once the payment process has been initiated, no changes to the sample analysis or procedures will be permitted unless deemed necessary for the analysis, as determined by the operator's discretion.
  5. Users are required to submit their samples to the specific lab where they wish to conduct their analysis 15 minute prior of their slot time. However, for XPS (X-ray Photoelectron Spectroscopy) analysis, users must submit their samples one day prior to the scheduled analysis date. This advance submission allows for the necessary preparations required for XPS analysis. Please ensure timely submission of samples according to these guidelines to facilitate smooth and efficient processing of your analysis requests.
  6. All preparation of samples as appropriate must be done at the user’s end prior to characterization. This may be discussed with the operator as deemed necessary.
  7. Radioactive materials, unstable substances, and explosive compounds must be reported to the facility. However, please note that for safety reasons, such samples may not be accepted for analysis due to potential hazards.
  8. The CIF facility or technical staff does not bear responsibility for the integrity of samples. The SIC's decision prior to analysis is final and must be adhered to by users.
  9. If user wish to retrieve their sample after the analysis, please inform the SIC before the analysis begins. However, please note that for certain analyses such as FESEM, XPS (X-ray Photoelectron Spectroscopy), Single Crystal XRD (X-ray Diffraction), Chemisorption, the sample may not be recoverable after the analysis is completed.
  10. Users are responsible for coordinating with the SIC before analysis and providing all relevant information related to the sample. Users should arrange to meet and discuss at a time convenient for the technical staff to ensure that ongoing sample analyses are not disrupted.
  11. Users are expected to provide information on how the results of the analysis have been utilized, such as in paper publications, theses, or other forms of dissemination. This feedback helps in understanding the impact and application of the analytical data
  12. Users must ensure they arrive on time for their scheduled slot as provided by the SIC. Please note that if there is a delay, the slot duration will not be extended. If you anticipate being unable to attend your scheduled slot, please inform the same in advance so that an alternative slot can be arranged for you. This will help ensure efficient use of resources and minimize disruptions to the schedule.
  13. Please be aware that additional charges may apply for special analysis requests that are not included in the standard list of charges for the respective equipment. If you have specific analysis requirements that go beyond the standard services provided, please discuss these with the operator beforehand to understand any associated extra costs that may be applicable. This will ensure transparency and clarity regarding pricing for the requested services.
  14. Users are expected to behave responsibly and refrain from abusing or shouting at the SIC. In case of any discrepancy or dispute with the SIC, users should contact the Faculty in-charge (cifservices@pilani.bits-pilani.ac.in) to resolve the issue before proceeding with the analysis.
  15. The charge list and policy will be reviewed periodically, and any changes will be communicated to all users by CIF (Central Instrumentation Facility). If you have any suggestions or notice any errors in this document, please bring them to the attention of the Faculty in-charge at ic.cif@pilani.bits-pilani.ac.in . Your feedback is valuable in maintaining the accuracy and effectiveness of our services. Thank you for your cooperation.

 

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