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Dr. Jagpreet Singh Sidhu

Assistant Professor, Department of Pharmacy

Cell Imaging, Disease Diagnosis, Fluorogenic Probes, Medicinal and Synthetic Organic Chemistry, Organic Hybrids
Department of Pharmacy, Birla Institute of Technology & Science, Pilani- 333031, Rajasthan. India.

Tracing Enzymatic Activities

Our research work focused on tracing enzymatic activity for diagnosis of diseased cells. Enzymes catalyze the most challenging and fascinating transformations in the living beings. Our research interest leverages the immense synthetic potential harbored in staggering inventory of enzymes to provide solutions to outstanding problems at the frontiers of chemistry and biology. Central to this research endeavor is to bring new concepts to biosensing catalysis by drawing inspiration from mechanistic connections between synthetic and biocatalytic systems. We developed interdisciplinary approaches that integrate organic synthesis, chemical biology, protein engineering, and biochemical analysis to sense reaction space beyond their current catalytic repertoire. These enzymes not only provide powerful tools for numerous synthetic and biological applications, but also offer a fertile ground to enrich our fundamental knowledge of biochemical reactions and enzymatic catalysis. These biochemical reactions were monitored using fluorescence spectroscopy.