Doctoral thesis entitled "Uptake and toxicity of copper in the diazotrophic cyanobacteriium Nostoc calcicola" mainly focuses on the parameters and kinetics of active uptake and the mechanism of metal toxicity in photoautotrophic microbe. The enzyme kinetics under heavy metal stress has also been studied.
Post-Doctoral work included two important aspects of cyanobacterial research-that is biological nitrogen fixation, herbicide resistance and genetic regulation of heavy metal resistance. We have reported for the first an energy- and light- dependent efflux of copper as the mechanism of metal resistance in cyanobacteria (FEMS Microbiol Let) and genetic transformation of glutamine prototrophy in cyanobacteria (Archieves Microbiol.).
Currently, leading a team of Post-Doc, PhD, and several M.Sc and M.E. students which is focusing on aspects related with biosensor development, aptamer technology, biodiesel production, bio sorption of heavy metal from industrial effluent. In the recently completed major research project funded by Department of Atomic Energy, we have developed a series of microbial mutants with increased metal accumulation. Attempts are being directed towards the optimization of process parameters for this purpose
Another project which was related with the development of recombinant-DNA based biosensor for the detection of antibiotics from the biological samples, we have cloned tetracycline sensitive promoter into a DsRed and GFP plasmids. Such strains have proved to be highly sensitive system to detect antibiotics at nanomole concentration. Similar work on metal and radioisotope biosensor is also in progress.
Completed a WIPO funded multi-centre project” Impact of IP system on economic growth” as country expert.
Supervised over 70 graduates and Master's project in the area of Environmental Biotechnology. Twelve students have been awarded Ph.D. degree.
Ongoing Research Projects
Aptamer based impedimetric sensor for detection of toxic metals in potable water
Completed Research Projects
Integrated approach for the efficient removal and recovery of heavy metal catalyst (Zinc) used in viscos fiber industries- ABSTCL, Mumbai
Bio-leaching of copper from low grade ore at Hindustan Copper Limited, Khetri, Union Ministry of Mines, New Dehli
Zinc Recovery from Industrial Waste using Bioleaching. Under Centre for Research Excellence, BITS Pilani
Development of recombinant DNA based Biosensor device for detection of arsenic in water samples, DST, New Delhi
Characterization and Pharmacological Evaluation of Bioactive Compound (Cyanotoxins) from the Cyanobacterial Isolates from Shekhawati Region of Rajasthan, UGC, New Delhi.
Bio-diesel production from microalgae, CSIR, New Delhi
Recombinant DNA based biosensor development for the detection of antibiotics – UGC major research project, New Delhi
Development of biosorbent technology for the treatment of radioactive waste –BRNS/ DAE, Mumbai.
Prokaryotic Vector Design using Cryptic Plasmids from Leuconostoc sp., Lactococcus species and Cyanobacteria, funded by Dabur Research Foundation, INDIA.
Impact of Intellectual Property system on Economic Growth. WIPO Japan
Development of Biosorption technology for the removal of copper from Khetri copper mine complex-UGC Minor research Project, New Delhi.
Fabrication and testing of a non-conventional machine for DNA amplification, - UGC Minor research Project, New Delhi
Development of herbicide resistant mutants of cyanobacteria- DST, Rajasthan.
Designing of a biofuel cell for hydrogen production- DST, Rajasthan